![]() ![]() The advantages of using this system in flow injection mode, particularly in terms of increased linear range, were also addressed. Practical detection limits (0.1 μM) were four–five orders of magnitude better for this system compared with a conventional electrode system. In terms of sensitivity, reproducibility, equilibration time and linear dynamic range, the performance of the microdisc based sensing system was superior to similar macroelectrode versions. Parameters affecting sensor performance were investigated in both batch and flow injection modes. The unique features of this system are demonstrated in this paper using the glucose - glucose oxidase (GOD) system. The result is an all in one generic electrochemical sensor that can be used in a wide range of biosensor applications in both batch or flow injection modes. The hydrophilic nature of the PU–PEO mixture ensures the biochemical moiety is trapped in a stable, hydrophilic environment. Enclosing the tip of the probe within a dialysis membrane completes the electrochemical cell. Immobilisation of the biochemical moiety onto the surface of the probe is achieved using a mixture of polyurethane (PU) and polyethylene oxide (PEO). ![]() Utilising a random array of platinum microdiscs as the working electrode, it incorporates a Ag/AgCl reference electrode and platinum auxiliary electrode into the tip of a polished glassy Teflon probe. A new type of electrochemical cell has been developed for use in biosensor applications.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
June 2023
Categories |